Biologics Tutorial: Work with Mixtures and Batches

Documentation
This tutorial works with media and media recipes. The tutorial shows you how to:
  • Register a new media a recipe (a "mixture").
  • Register a batch of the new recipe.

Register a Mixture

Mixtures are designed to register lab recipes and formulations. Their components are ingredients and protocol descriptions.

  • Go to Media > Mixture.
  • Select Create > Mixture.
  • On the Details tab, enter the following:
    • Mixture Name: PBS - Cold Spring
    • Description: Cold Spring formumlation with optional ingredients.
    • Alias: cold spring
    • Mixture Type: Solution
    • Click Next.
  • On the Ingredients tab, enter the following:
    • Recipe Measure Type: Volume
    • Expiration Time: 90 Days
    • Ingredients: Known
    • Click Bulk Upload.
    • In the popup dialog, copy-and-paste the follow recipe into the text box. Click Add Ingredients to submit the recipe.
TypeIngredient/MixtureAmountUnit Type
IngredientSodium Chloride80g/L
IngredientPotassium Chloride2g/L
IngredientSodium Phosphate Dibasic Anhydrous14.4g/L
IngredientPotassium Phosphate Monobasic2.4g/L
IngredientCalcium Chloride, Dihydrate, Granular1.33g/L
IngredientMagnesium chloride, Anhydrous1g/L
    • Click Next.
  • On the Preparation tab:
    • Click Add Stop.
    • In Step Description enter the following:
Dissolve the reagents listed above in 800 mL of H2O. Adjust the pH to 7.4 (or 7.2, if required) with HCl, and then add H2O to 1 L. Dispense the solution into aliquots and sterilize them by autoclaving for 20 min at 15 psi (1.05 kg/cm2) on liquid cycle or by filter sterilization. Store PBS at room temperature.
    • Click Next.
  • On the Confirm tab:
    • Review the entries and click Finish.

Create a Batch

  • In the grid, place a checkmark next to PBS - Cold Spring.
  • Select Create > Media > Batch.
  • On the Details tab, enter the following:
    • Mixture Used: PBS - Cold Spring
    • Batch Name: "Batch for Project H", or leave this field blank and Biologics will generate a batch name.
    • Description: 'Progress evaluation of Project H'.
    • Vendor/Part No. : none
    • Consumed: leave unchecked
  • On the Preparation tab, enter the following:
    • Desired Batch Yield: 1
    • Use the dropdown to select: "kg"
    • Actual Batch Yield: 1
    • Expiration Date: Note that this has been auto-filled, based on the Mixture's expiration property.
    • Materials/Ingredient Source: Known
    • Amounts/Raw Materials: Known
    • Click Bulk Upload.
    • Copy-and-paste the following materials list into the text box, overwriting the pre-populated text. Click Add Ingredients to submit the list.
IngredientAmount UsedRaw Material Used
Sodium Chloride80RM-20181129-52
Potassium chloride2unknown
Sodium phosphate dibasic anhydrous14.4RM-20181129-53
Potassium Phosphate monobasic2.4RM-20181129-51
Calcium Chloride, Dihydrate, Granular1.33RM-20181129-6
Magnesium chloride, anhydrous1unknown
    • Notice that some ingredients refer to specific lot numbers already known to the registry, while others have 'unknown' sources.
    • Click Next.
  • On the Confirm tab, click Finish.
  • A details page for the new batch is displayed.
  • Click Batches
  • A table of batches is shown, including the new batch added in this tutorial.

Tutorial Curriculum

Biologics Tutorial: Register Samples and Experiments

Biologics Tutorial: Create a New Biologics Project

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