This tutorial works with media and media recipes. The tutorial shows you how to:

• Register a new media mixture. In the Biologics terminology, a "mixture" is a recipe, i.e., instructions for mixing together ingredients. A mixture is a virtual entity, not a physical thing that you can place in a bottle.
• Register a physical batch of the mixture. In the Biologics terminology, a "batch" is a physical thing, i.e., the physical result of following the instructions in a recipe/mixture.

Register a Mixture

Mixtures are designed to register lab recipes and formulations. Their components are ingredients and protocol descriptions.

• From the main menu, under Media, click Mixtures.
• Click Add.

• On the Details tab, enter the following:
• Mixture Name: PBS - Cold Spring
• Description: Cold Spring formulation with optional ingredients.
• Alias: cold spring
• Mixture Type: Solution
• Click Next.

• On the Ingredients tab, enter the following:
• Recipe Measure Type: Volume
• Expiration Time: 90 Days
• Ingredients: Known
• Click Bulk Upload.

• In the pop-up dialog, copy-and-paste the following recipe into the text box.

• Notice that you could either append these ingredients to any you'd already entered or replace any existing ingredients.
• Click Add Ingredients to submit the recipe.

• Click Next.

• On the Preparation tab:
• Click Add Step.
• In Step Description enter the following:

  Dissolve the reagents in 800 mL of H2O. Adjust the pH to 7.4 (or 7.2, if required) with HCl, and then add H2O to 1 L. Dispense the solution into aliquots and sterilize them by autoclaving for 20 min at 15 psi (1.05 kg/cm2) on liquid cycle or by filter sterilization. Store PBS at room temperature.

• You could of course split this procedure into multiple steps, but that is unnecessary for this tutorial.

• Click Next.
• On the Confirm tab:
• Review the entries and click Finish.

Create a Batch

• Select Batches from the main menu or the tabs at the top of the page.
• Click Add.
• On the Details tab, enter (or select) the following:
• Mixture Used: PBS - Cold Spring
• Batch Name: Enter "Batch for Project H", or leave this field blank and Biologics will generate a batch name for you.
• Alias: Leave blank.
• Description: Enter "Progress evaluation of Project H"
• Vendor/Part No. : Leave blank.
• Consumed: Leave unchecked.
• Click Next.
• On the Preparation tab, enter the following:
• Desired Batch Yield: 1
• Use the dropdown to select: "L"
• Actual Batch Yield: 1
• Expiration Date: Note that this has been auto-filled, based on the Mixture's expiration property.
• Materials/Ingredient Source: Known
• Amounts/Raw Materials: Known
• Click Bulk Upload.

• Copy-and-paste the following materials list into the text box, overwriting the pre-populated text. For simplicity, we have used 'unknown' raw ingredients instead of specific lot numbers already known to the registry.

• Click Add Ingredients to submit the list.

• Notice that the slider for Amounts/Raw Materials changed to Unknown.
• Click Next.

• On the Confirm tab, click Finish.
• A details page for the new batch is displayed.

• Click Batches

• A table of batches is shown, including the new batch added in this tutorial.

Tutorial Curriculum

Biologics Tutorial: Register Samples

Biologics Tutorial: Create a New Biologics Project