Premium Feature — Available with LabKey Biologics LIMS. Learn more or contact LabKey.

Many entity types can be uploaded in bulk using any common tabular format. Protein and nucleotide sequences can be imported using the GenBank format.

Upon upload, the registry will calculate and create any molecular species and sets as appropriate.

Supported File Formats

The file formats supported are listed in the file import UI under the drop target area.

Tabular file formats are supported for all entity types:

  • Excel: .xls, .xlsx
  • Text: .csv, .tsv
Nucleotide sequences, constructs and vectors can also be imported using GenBank file formats:
  • GenBank: .genbank, .gb, .gbk
LabKey Biologics parses GenBank files for sequences and associated annotation features. When importing GenBank files, corresponding entities, such as new nucleotide and protein sequences, are added to the registry.

Assemble Bulk Data

When assembling your entity data into a tabular format, keep in mind that each Registry Source Type has a different set of required column headings.

Indicate Lineage Relationships

Lineage relationships (parentage) of entities can be included in bulk registration by adding "DataInputs/<DataClassType>" columns and providing parent IDs.

For example, to include a Vector as a 'parent' for a bulk registered set of Expression Systems, after obtaining the template for Expression Systems, add a new column named "DataInputs/Vector" and provide the parent vector name for each row along with other fields defining the new Expression Systems.

Bulk Upload Registry Source Data

After you have assembled your information into a table, you can upload it to the registry:

  • Go the Registry Source Type you wish to import.
  • Select Add > Import from File.
  • On the import page, you can download a template if you don't have one already, then populate it with your data.
  • Confirm that the Registry Source Type you want is selected, then drag and drop your file into the target area and click Import.

If you want to update existing registry sources or merge updates and creation of new sources, use Edit > Update from File.

Bulk Data Example Files

Example Nucleotide Sequence File

Notes:

  • Annotations: Add annotation data using a JSON snippet, format is shown below.
namealiasdescriptionflagprotSequencessequenceannotations
NS-23Signal Peptide 1An important sequence.FALSE[{name: "PS-23"}]CCCCTCCTTG
GAGGCGCGCA
ATCATACAAC
CGGGCACATG
ATGCGTACGC
CCGTCCAGTA
CGCCCACCTC
CGCGGGCCCG
GTCCGAGAGC
TGGAAGGGCA
[  
{
name:"First Annotation",
category:"Feature",
type:"Leader",
start:1,
end:20
},
{
name:"Another Annotation",
category:"Feature",
type:"Constant",
start:30,
end:50
}
]

When importing the rows for NucSequence, you can reference the corresponding ProtSequence and the translation start, end, and offset. (The offsets are 1-based.) An example:

namealiasdescriptionflagprotSequencessequenceannotations
NS-100Signal Peptide 1some descriptionFALSE[{name: "PS-100", nucleotideStart=1, nucleotideEnd=30, translationFrame=2}]ATGGAGTTGGGACTGAGCTGGATTTTCCTTTTGGCTATTTTAAAAGGTGTCCAGTGT 

Example Protein Sequence File

Notes:

  • Organisms: A comma separated list of applicable organisms. The list, even if it has only one member, must be framed by square brackets. Examples: [human] OR [human, rat, mouse]
  • ?: The column header for the extinction coefficient (ε).
  • %?: The column header for the % extinction coefficient ().
NameAliasDescriptionNuc SequencesChain FormatAvg. MasspI?%?Num. S-SNum. CysOrganismsSequence
PStest-150 Test sequence for import 113999.648.030355002.5412[mouse, rat]EVQLVESGEL
IVISLIVESS
PSSLSGGLVQ
GGGSLRLSCA
ASGELIVISL
IVESSPSSLS
YSFTGHWMNW
VRQAPGKGLE
WVGIMIHPSD
SETRYNQKFK
DELIVISLIV
ESSPSSLSIR
FTISVDKSKN
TLYLQMNSLR
AEDTAVYYCA
RIGIYFYGTT
YFDYIWGQGT

Mixtures and Batches

The text 'unknown' can entered for certain fields. For Mixtures, the Amount field; for Mixture Batches, the Amount and the RawMaterial fields.

Mixture Bulk Upload

TypeIngredient/MixtureAmount Unit Type
IngredientI-2unknown

Batch Bulk Upload

IngredientAmount UsedRaw Material Used
Sodium phosphate dibasic anhydrous5RawMat-1234
Sodium Chlorideunknownunknown
Potassium chlorideunknownunknown

Downloadable Example Files

Download a sample file for a given entity type:

Related Topics

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