Vectors, Constructs, Cell Lines, and Expression Systems

2024-03-29

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The LabKey Biologics registry can capture Vectors, Constructs, Cell Lines, Expression Systems, and their relationships. To add these entities to the registry, use the creation wizard for the desired type. Creation in bulk via file import is also available.

Definitions

  • Vectors are typically plasmids that can inject genetic material into cells. Must have a specific nucleotide sequence.
  • Constructs are Vectors which have been modified to include the genetic material intended for injection into the cell.
  • Cell Lines are types of cells that can be grown in the lab.
  • Expression Systems are cell lines that have been injected with a construct.

Add Manually within the User Interface

Select the desired entity from the main menu, then select Add > Add manually.

The default fields for each entity type are shown below. General instructions for creating entities are in this topic: Create Registry Sources

Note that the default fields can be changed by administrators to fit your laboratory workflows, requirements, and terminology. For details about customizing them, see Biologics: Grids, Detail Pages, and Entry Forms.

Entity TypeDefault Fields
VectorName
Common Name
Description
Alias
Sequence
Selection Methods
Vector Parents
ConstructName
Common Name
Description
Alias
Vector
Cloning Site
Complete Sequence
Insert Sequences
Construct Parents
Cell LinesName
Common Name
Description
Alias
Expression System
Stable
Clonal
Organisms
Cell Line Parents
Expression SystemsName
Common Name
Description
Alias
Host Cell Line
Constructs
Expression System Parents

Add Manually from Grid

Select the desired entity from the main menu, then select Add > Add Manually from Grid. Enter as many rows populated with values for the necessary fields.

Learn about creating entities with this kind of grid in the topic:

Import from File

Creation of many entities of a given type can also be done in bulk via file import using a template for assistance. Select Add > Import from File and upload the file. Learn more in this topic:

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