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Skyline-reading Agilent MRM with multiple scan segments

LabKey Support Forum

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Skyline-reading Agilent MRM with multiple scan segments weigandm  2024-09-23 06:29
Status: Active
 

Hello,

I collected MRM transitions on an Agilent QqQ, I used 2 scan segments. One scan segment was from 0-1.1mins, during this time solvent was sent to waste, then from 1.1 to end solvent was sent to MS for detection. When I upload the .d file into Skyline, it is only finding the 0-1.1 min time segment when there would be nothing. How do I get Skyline to find or read the other time segment where there is actually data?
 
 
mohara responded:  2024-09-23 09:27

Hello:
I was going to direct you to the Skyline support site, where I can see that you've already asked this question. There also looks to be a similar sounding question already open.
Hopefully the team there can assist you.
--Molly