) with flow data and assign additional meanings to keywords.
Additional information about groups of FCS files can be uploaded in spreadsheet and associated with the FCS files using keywords.
- To upload the data, go to the flow dashboard and click Upload Sample Descriptions.
- Then copy/paste the sample information into the text box, or upload a file directly.
In the
sample data download available here:
Step 1: Set Up a Flow Folder, you can find the file "labkey-flow-demo-123/labkey-demo/Workspaces/sample-set.tsv".
Sample set uploads must be formatted as
tab separated values (TSV). The first row should contain column names, and subsequent rows should contain the data.
- After pasting in the data or choosing the file, you'll need to identify which column(s) make each sample unique.
- In this case, the "AssayId" and "SampOrd" columns are the uniquely identifying columns for each sample.
- Select a parent column if needed.
Once you've uploaded the sample information, you need to associate the sample columns with the FCS file's keywords using the sample join fields.
- Click Flow Dashboard.
- Click Define sample description join fields and specify the join as:
Sample Property | FCS Property |
---|
"AssayId" | "EXPERIMENT NAME" |
"SampOrd" | "Sample Order" |
You will now see a new column in the
FCSFile table.
- Click Flow Dashboard.
- Click on FCS Files in the Flow Summary on the right.
- Click FACSData.
- Then click Grid Views > Customize Grid to open the grid customizer.
- You should now see the columns from the Sample table that you may add to your grid.