Tutorial: ELISA Assay

This tutorial shows you how to:

Set up an ELISA Plate Template and Assay Design

To begin, create an assay folder to work in:

Next configure a plate template that corresponds to the plate of your ELISA experiment.

  • In the Assay List web part, click Manage Assays.
  • Click Configure Plate Templates.
  • Select "New 96 well (8X12) ELISA default template".
  • Enter a Template Name, for example, "ELISA Plate 1".
  • Review the shape of the template, clicking the Control, Specimen, and Replicate tabs to see the well groupings. Notice that you could edit the template to match alternate well groupings if needed; instructions can be found in Edit Plate Templates. For the purposes of this tutorial, simply save the default.
  • When finished, click Save & Close.

Now you can create the assay design using that template:

  • Click the ELISA Experiment link to return to the main folder page.
  • Click New Assay Design
  • Select ELISA and choose "Current Folder (ELISA Experiment)" from the Assay Location dropdown, then click Next.
  • Provide a Name, for example, "HIV-ENV1 - ELISA Assay".
  • Select a Plate Template: "ELISA Plate 1" (it may already be selected by default).
  • Scroll down the page and review the data fields. You could add or edit fields, but for this tutorial, leave the default design unchanged.
  • Click Save & Close.
  • You will return to the main folder page and see your new design on the assay list.

Import ELISA Experiment Data

  • Download sample data:
  • Return to the main ELISA Experiment folder page.
  • In the Assay List, click the name you gave your assay design, here: HIV-ENV1 - ELISA Assay.
  • Click Import Data.
  • Accept the default batch properties by clicking Next.
  • In the Run Data field, click Browse or Choose File and select one of the sample data files you just downloaded. Make no other changes on this page, then click Next.
  • On the next page, enter Concentrations for Standard Wells as shown here:
  • Click Save and Import Another Run. Repeat the import process for the other two files, entering the other two Run Data filenames. The concentrations for standard wells will retain their values so you do not need to reenter them. Clicking Save and Finish after the last file.

Visualize the Data

When you are finished importing, you'll see the runs grid showing the files you just uploaded. Since you did not specify any other Assay IDs, the filenames are used. Browse the data and available visualizations:

  • Hover over the row for Assay Id "biotek_01.xls", click (Details).
  • You will see a visualization and tabular grid of the data. The server automatically generates a calibration curve for the control values.

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